01882nas a2200229   4500008004100000020001400041245011400055210006900169300001000238490001000248520121400258653001401472653001501486653001101501653001201512653001601524653001001540653002001550100002101570700001701591856004401608       2010                        eng d  a1755-099800aTissue segregation of mitochondrial haplotypes in heteroplasmic Hawaiian bees: implications for DNA barcoding0 aTissue segregation of mitochondrial haplotypes in heteroplasmic   a60-680 v10(1)3 aThe issue of mitochondrial heteroplasmy has been cited as a theoretical problem for DNA barcoding but is only beginning to be examined in natural systems. We sequenced multiple DNA extractions from 20 individuals of four Hawaiian Hylaeus bee species known to be heteroplasmic. All species showed strong differences at polymorphic sites between abdominal and muscle tissue in most individuals, and only two individuals had no obvious segregation. Two specimens produced completely clean sequences from abdominal DNA. The fact that these differences are clearly visible by direct sequencing indicates that substantial intra-individual mtDNA diversity may be overlooked when DNA is taken from small tissue fragments. At the same time, differences in haplotype distribution among individuals may result in incorrect recognition of cryptic species. Because DNA barcoding studies typically use only a small fragment of an organism, they are particularly vulnerable to sequencing bias where heteroplasmy and haplotype segregation are present. It is important to anticipate this possibility prior to undertaking large-scale barcoding projects to reduce the likelihood of haplotype segregation confounding the results.10abarcoding10aColletidae10aHAWAII10aHylaeus10aHYMENOPTERA10amtDNA10aPacific Insects1 aMagnacca, K., N.1 aBrown, M.J.F  uhttps://pacleps.myspecies.info/node/128