Isolation and characterization of microsatellite markers in an endemic Hawaiian planthopper (Nesosydne chambersi: Delphacidae)

Publication Type:Journal Article
Year of Publication:2008
Authors:K. R. Goodman, Morris, V. R. F., Welter, S. C., Roderick, G. K.
Journal:Molecular Ecology ResourcesMolecular Ecology ResourcesMolecular Ecology Resources
Volume:8
Pagination:1436-1438
Date Published:Nov
Type of Article:Article
ISBN Number:1755-098X
Accession Number:ISI:000260528700065
Keywords:Delphacidae, HAWAII, Heteroptera, microsatellite, Nesosydne, Pacific Insects, planthopper, SSR
Abstract:

We have isolated and characterized 17 microsatellite loci for the endemic Hawaiian planthopper Nesosydne chambersi (Delphacidae), a member of a large Hawaiian Nesosydne radiation. Thirty individuals from one population and 10 individuals from two populations across the species' range were tested to investigate polymorphism. The observed loci contained two to nine alleles per locus. Expected heterozygosity within this species ranged from 0.2 to 0.85. These markers will be used to assess intraspecific differentiation and population structure within N. chambersi.

Short Title:Mol. Ecol. Resour.Mol. Ecol. Resour.
Alternate Journal:Mol. Ecol. Resour.
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